ACID FAST DECOLOURIZER |
| Article No. | 80255 | Grade | Laboratory Reagent | |
| Synonyms | — | C.I. No. | — | |
| CAS No. | — | Purity | — | |
| Molecular Formula | — | Molecular Weight | — | |
| H.S. Code | 38220090 | Shelf Life | 36 Months | |
| Physical Properties | ||||
| Physical state at 20 °C | Liquid | Colour | Colourless | |
| Solubility in water [% weight] | Miscible with water. | |||
| Specifications Parameters | ||||
| Description | A clear and colourless liquid. | |||
| Suitability test | Passes test | |||
| Directions | 1. Prepare a smear on a clear, dry glass slide.
2. Allow it to air dry and fix with gentle heat. 3. Flood the smear with Carbol Fuchsin stain. Heat to steaming for 5 minutes with a low flame; do not boil the stain and do not permit drying of the smear. 4. Allow it to stand for 5 minutes without further heating. 5. Wash in the running tap water. 6. Decolourizer with Acid Fast Decolourizer for 2 minutes or until no more stain comes off in the washings. (If washing is not thorough, may get false positive results). 7. Wash with the tap water. 8. Counterstain for 30 seconds with Methylene Blue. Wash with tap water, dry in air, then examine under an oil immersion objective. |
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| Results | Acid fast organisms: Bright red
All other organisms and background material: Blue |
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| Intended Use | Acid fast decolourizer is used as decolorizing solution for Acid Fast staining. | |||
| Principle And Interpretation | Mycobacteria (AFB/Acid Fast Bacteria) are difficult to stain due to high lipid and wax content in their cell walls. Acid fast is a differential staining technique useful for identification of the tubercle bacillus, other Mycobacteria, and Nocardia, which depends on the chemical composition of the bacterial cell wall. It is also called as Ziehl-Neelsen staining technique. Because of the difficulty in staining these organisms with ordinary dyes, basic dyes in the presence of controlled amounts of acid are used. The primary stain used in acid-fast staining, carbol fuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. Generally, heat must be applied during the staining procedure, or wetting agents must be used, to aid dye penetration. Organisms exhibiting the property of acid fastness, once stained, are not easily decolourized by alcohol. Non-acid fast organisms are decolourized by acid fast decolourizer and take up the counter stain. | |||
| Note(s): Assay (if applicable) method mentioned.
WARNING Hazard statements: May cause respiratory irritation. Flammable liquid and vapour. Harmful if swallowed. Causes mild skin irritation. Causes eye irritation. PRECAUTIONARY STATEMENTS : Prevention: Ground/bond container and receiving equipment. Use only non-sparking tools. Take precautionary Measures against static discharge. Wash hands thoroughly after handling. Do not eat, drink or smoke when using this product. Keep away from heat/sparks/open flame – No smoking. Keep container tightly closed. Use explosion-proof electrical/ventilating/lighting/equipment. Wear protective gloves and eye/face protection. Response: If eye irritation persists, get medical advice/attention. If skin irritation occurs, seek medical advice/attention. IF SWALLOWED: Rinse mouth. Do NOT induce vomiting. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. Specific treatment: refer to Label or MSDS. If on skin or hair: remove/take off immediately all contaminated clothing. Rinse with water/shower. In case of fire, use foam for extinction. |
IMDG Code : 3/II
UN No. : 1170 IATA : 3 |
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| Disposal: Allow to evaporate in safe open area (applicable for small quantities). The chemical should be burnt in a chemical incinerator equipped with burner and scrubber. If the volumes are larger than 500ml and no incinerator and site of evaporation is not available then transfer the waste chemical to waste solvent drum for collection by specialist disposal company. | ||||
| Hazard Pictogram(s) : GHS02, GHS07 | ||||
| Manufacturer | Auraiya Laboratory Chemicals Pvt Ltd | Net Packing | 100ml, 500ml |
| Country of Origin | India | Website | www.auraiyalabchem.com |
| Item model number | 80255 | Order now | sales@auraiyalabchem.com |
| Packer | Auraiya Laboratory Chemicals Pvt Ltd | Contact us | +91 879 151 1893 |
| Importer | Auraiya Laboratory Chemicals Pvt Ltd | Customer Care | info@auraiyalabchem.com |
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