NEWMAN’S STAIN (MODIFIED)FOR MICROSCOPICAL STAINING |
| Article No. | 86140 | Grade | For Microscopical Staining | |
| Synonyms | — | C.I. No. | — | |
| CAS No. | — | Purity | — | |
| Molecular Formula | — | Molecular Weight | — | |
| H.S. Code | 38220090 | Shelf Life | 36 Months | |
| Physical Properties | ||||
| Physical state at 20 °C | Liquid | Colour | Dark red | |
| Solubility in water [% weight] | Miscible with water. | Odour | Odourless | |
| Specifications Parameters | ||||
| Description | Dark blue coloured solution. | |||
| Suitability for bacteria & bovine cell in milk | Passes test | |||
| Directions | 1. Use clean glass slides for smear preparation. For quantitative determination of number of organisms, a measured quantity (0.01 ml) of the dairy product (or a known dilution of it) is evenly spread over a 1 sq.cm area of the glass slide. The smear should be rapidly dried at around 45-50°C. Cool it to room temperature.
2. Submerge slides of the fixed, dried films, singly or in multiples, into the stain for 2 minutes. 3. Drain off the excess stain by resting the edge of the slide on absorbent paper. 4. Dry the slides thoroughly (by forced air if available). 5. Rinse the dried stained slides thrice in water at 35°C to 45°C and then drain and air dry before examining the film under the microscope. 6. Examine the film on the slide under an oil-immersion objective after placing one drop of immersion oil on the film. 7. Enumerate the number of cells per ml of dairy product under study. |
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| Results | Bacteria: Blue cells observed.
Somatic cells: Blue with distinct nuclear lobes observed. |
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| Intended Use | Newman’s Stain, Modified is used for staining of bacteria and somatic cell count in milk or certain milk products | |||
| Principle And Interpretation | It is based on the examination of stained thin film of a measured volume of milk spread over a specified area on a glass slide. The method is useful for rapid estimation of the total bacterial population (including live and dead cells) of a sample of milk. In this test, milk smear is prepared on one square centimeter area. The smear is stained with Newman’s stain and examined under microscope. Each microscopic field examined represents a quantitative aliquot of the milk sample. The number of microscopic fields occurring in one square centimeter area of the milk smear will vary as the diameter of the microscopic field varies with different microscope. | |||
| Note(s): Assay (if applicable) method mentioned.
WARNING Hazard statements: Not hazardous. No hazards. Precautionary statements Prevention: —————————————— Response: ———————————————— |
IMDG Code :
UN No. : IATA : |
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| Disposal: —————– | ||||
| Hazard Pictogram(s) : – | ||||
| Manufacturer | Auraiya Laboratory Chemicals Pvt Ltd | Net Packing | 100 ml |
| Country of Origin | India | Website | www.auraiyalabchem.com |
| Item model number | 86140 | Order now | sales@auraiyalabchem.com |
| Packer | Auraiya Laboratory Chemicals Pvt Ltd | Contact us | +91 879 151 1893 |
| Importer | Auraiya Laboratory Chemicals Pvt Ltd | Customer Care | info@auraiyalabchem.com |
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